Products

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3T3 fibroblasts are used as feeder cells in culturing human keratinocytes for use in a variety of applications, including:

• Skin gene therapy
• Skin grafts
• Wound healing research

The Waisman Clinical Biomanufacturing Facility (WCBF) is now offering irradiated 3T3 feeder cells that have been produced and tested according to cGMP guidelines. These cells will be provided to researchers on a non-profit basis under a contract from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) to support skin gene therapy and wound healing clinical trials.

Product Specifications: Cells per vial: >3 X 106 Plating Efficiency: 1 vial will seed a P100 culture dish to > 30% confluence Endotoxin: < 10 EU /ml Sterility (as per 21 CFR 610.12): No growth Mycoplasma: Negative Cryopreservation Media: 10-20% bovine serum and 10% DMSO in DMEM (1 ml)

Ordering Information

Cost: 1 Vial - $97 (plus S&H)

To order: Please download a Cell request form and a User-WCBF Material Transfer Agreement (MTA).

• Cell request form
• User-WCBF Material Transfer Agreement (MTA)

The agreements below are copies of agreements between the WCBF and the original cell line sources as referenced in the above MTA.

• NIH Cell Line (MTA NIH.pdf)
• WCBF MTA J2 Cell Line (WCBF MTA J2.pdf)