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Cellular and Molecular Neuroscience Core
Introduction:
This core responds to a growing demand for services and
specialized equipment needed to conduct studies at the cellular and
molecular levels on human and animal tissues. The services include
training in use of advanced techniques and equipment including
confocal and fluorescence imaging, stereology, FACS analysis, and
gene chip and real-time PCR quantitation of mRNA levels. In
addition, the core developed a new viral vector production
service, with a focus on lentiviruses, to facilitate gene transfer
studies in cell culture and in animal models.
Objectives:
The objectives of the CMN Core are to:
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Provide high
quality, cost effective, cellular and molecular neuroscience
equipment, technology and expertise to IDDRC investigators. The
CMN Core provides access to, and staff support for brightfield,
fluorescence and confocal microscopy, stereology software, flow
cytometry, and phosphorimaging, as well as specialized molecular
technology including real-time PCR, DNA microarray analysis,
2-dimensional gel electrophoresis, mass spectrometry, and DNA
synthesis and sequencing.
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Provide access to small or large-scale viral vector design and
production of plasmids for in vitro or in vivo gene delivery.
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Provide training and educational seminars to familiarize
investigators with new technology/approaches.
Staff:
Link to CMN Web Site:
Access to Core Services:
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